On 23 Oct 1575 John married Alice Lambert in Sherburne, Dorset. Alice died in Semley, Wiltshire ca 1623.

Will of Alice Hayne, widow, of Semley, Wiltshire, dated 2 Mar 1620/1. Proved by Walter Haynes, March 2, 1623/4. Mentions daughter Elizabeth Read, daughter Rose and son-in-law Andrew Ivie, son Walter, his daughters Elizabeth, Suffraine & Marie, his son Thomas.
    New England Hist. Gen. Reg. 39, page 263

The will is transcribed on pages 10–12 of Walter Haynes of Sutton Mandeville.18