On 16 May 1771 John married Bathsheba Shurtleff (48537) , daughter of Joseph Shurtleff (22 Jan 1715/6-1755) & Sarah Nelson (31152) (28 Mar 1716-31 Mar 1809), in Kingston, MA. Born on 11 May 1753. Bathsheba was baptized in Plymouth, MA, on 31 May 1753. Bathsheba died in Kingston, MA, on 19 Apr 1812; she was 58.

Children of John and Bathsheba (Shurtleff) Bartlett:
    i. Rufus Bartlett, b. 1771;
    ii. Dorothy Bartlett, b. 1774;
    iii. Sarah Bartlett, b. 1776, m. Simeon Chandler;
    iv. Betsey Bartlett, b. 1779, m. Bradford Holmes;
    v. Olive Bartlett, b. 1783, m. Thomas Bates;
    vi. John Bartlett, b. 1786;
    vii. George Bartlett, b. 1789;
    viii. Bathsheba Bartlett, b. 1793, m. Lazarus Drew; and
    ix. Joseph Bartlett.132